Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.

Generating a synthetic genome by whole genome assembly: X174 bacteriophage from synthetic oligonucleotides

We have improved upon the methodology and dramatically shortened the time required for accurate assembly of 5to 6-kb segments of DNA from synthetic oligonucleotides. As a test of this methodology, we have established conditions for the rapid (14day) assembly of the complete infectious genome of bacteriophage X174 (5,386 bp) from a single pool of chemically synthesized oligonucleotides. The proc...

Whole-Genome Sequencing of a Clinically Isolated Antibiotic-Resistant Enterococcus faecium EntfacYE

Background and Objective: Enterococcal infections are considered the most common nosocomial infections. Nowadays, enterococci show high resistance to common antibiotics, especially vancomycin. Vancomycin-resistant Enterococcus faecium is one of the most common nosocomial infections, which is included in the World Health Organization priority pathogens list for research and development of new an...

Optimal clone identifier for genomic shotgun libraries: "OC Identifier tool".

In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of putting thousands of gene sequences into a single slide allows the use of s...

Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides.

We have improved upon the methodology and dramatically shortened the time required for accurate assembly of 5- to 6-kb segments of DNA from synthetic oligonucleotides. As a test of this methodology, we have established conditions for the rapid (14-day) assembly of the complete infectious genome of bacteriophage X174 (5386 bp) from a single pool of chemically synthesized oligonucleotides. The pr...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System